Technical Data
A1355-64D
ALIX (Apoptosis-linked Gene 2 Interacting Protein X, ALG-2-interacting Protein 1, AIP1, Dopamine Receptor Interacting Protein 4, DRIP4, HP95, KIAA1375, MGC17003, Programmed Cell Death 6 Interacting Protein, PDCD6 Interacting Protein, PDCD6IP)
Description:
Alix (ALG-2-interacting protein X) is an adaptor protein that was first described for its capacity to bind to the calcium-binding protein ALG-2, the expression of which seemed necessary for cell death. Predicted molecular weight approximately 96kD. Alix contains an N-terminal Bro1 domain and a C-terminal proline-rich domain (PRD). The PRD contains multiple polyproline motifs, which are potential docking sites for proteins containing SH3 domains, interact with multiple cellular Alix-binding partners that are involved in apoptotic induction, endosomal sorting, and endocytosis. Bro1 domain interacts with CHMP4b which is also involved in endosomal sorting.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k3A9Affinity Purified
SizeStorageShippingSourceHost
25ug-20CBlue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant corresponding to full-length human Alix.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human Alix. Species Crossreactivity: mouse and frog.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wu Y, et al. 2000. Differentiation 67:139. 2. Missotten M, et al. 1999. Cell Death Differ. 6:124. 3. Odorizzi G. 2006. J Cell Sci. 119:3025.