Technical Data
A1355-82
ALKBH2, Recombinant, Human, aa1-261, His-tag (ABH2, Oxy DC1)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Alpha-ketoglutarate-dependent dioxygenase alkB homolog 2, also known ALKBH2, belongs to the ALKB family. Expressed in heart, colon, liver, testis, ovary, prostate and small intestine, ALKBH2 uses iron as a cofactor and functions as a dioxygenase that catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine. ALKBH2 is functionally activated by ascorbate and requires oxygen and a-ketoglutarate for enzymatic activity.

Source:
Recombinant corresponding to aa1-261 from human ALKBH2, fused to His-tag at N-terminus expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR
YKDGCDHIGE HRDDERELAP GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV RKKVLAPRVN LTFRKILLTK K

Molecular Weight:
~31.4kD (281aa), confirmed by MALDI-TOF

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~31.4kD
Source: E. coli
Purity: ~90% (SDS-PAGE)
Concentration: ~0.5mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 1mM DTT, 30% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Rinqvoll J., et al. (2008) Cancer Res. 68:4142-4149. 2. Lee D H., et al. (2005) J Biol Chem. 280:39448-39459

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.