Technical Data
A1355-83C
ALKBH3, Recombinant, Human, aa1-286, His-tag (Alpha-ketoglutarate-dependent Dioxygenase alkB Homolog 3, Alkylated DNA Repair Protein alkB Homolog 3, Prostate Cancer Antigen 1, DEPC-1, DEPC1, ABH3)
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
ALKBH3 is a member of the ALKB family of proteins and functions as a dioxygenase with a preference for RNA and single stranded DNA substrates. This protein repairs 1-methyladenine and 3-methylcytosine lesions in alkylated DNA and RNA and its activity is stimulated by ascorbate. ALKBH3 is expressed in a wide variety of tissues and localizes to the cytoplasm and the nucleus.

Source:
Recombinant corresponding to aa1-286 from human ALKBH3, fused to His-tag at N-terminus expressed in E.coli.

AA Sequence:
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELDM EEKRRRARVQ GAWAAPVKSQ AIAQPATTAK SHLHQKPGQT WKNKEHHLSD REFVFKEPQQ VVRRAPEPRV IDREGVYEIS LSPTGVSRVC LYPGFVDVKE ADWILEQLCQ DVPWKQRTGI REDITYQQPR LTAWYGELPY TYSRITMEPN PHWHPVLRTL KNRIEENTGH TFNSLLCNLY RNEKDSVDWH SDDEPSLGRC PIIASLSFGA TRTFEMRKKP PPEENGDYTY VERVKIPLDH GTLLIMEGAT QADWQHRVPK EYHSREPRVN LTFRTVYPDP RGAPW

Molecular Weight:
~37.9kD (325aa), confirmed by MALDI-TOF

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~37.9kD
Source: E.coli
Purity: ~95% (SDS-PAGE)
Concentration: ~1mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 0.1M sodium chloride, 2mM DTT, 10% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Ougland R., et al. (2004) Mol Cell. 16(1):107-16. 2. Duncan T., et al. (2002) Proc Natl Acad Sci U S A. 99(26):16660-5.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.