Technical Data
A1359-75
ALOX5 (5-lipoxygenase, 5-LO, 5LPG, Arachidonate 5-lipoxygenase, LOG5)
Description:
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:30,000
Western Blot: ~85kD
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide, C-ERNKKKQLPYYYLSPD, representing .the C Terminus of the human protein according to NP_000689.1
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.09% sodium azide.
Specificity:
Recognizes human ALOX5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Kalayci O, Birben E, Sackesen C, Keskin O, Tahan F, Wechsler ME, Civelek E, Soyer OU, Adalioglu G, Tuncer A, Israel E, Lilly C. ALOX5 promoter genotype, asthma severity and LTC production by eosinophils.Allergy. 2006 Jan;61(1):97-103. PMID: 16364163