Technical Data
Arachidonate 5-lipoxygenase (ALOX5, 5-LO, LOG5, 5-lipoxygenase)
ALOX5 is a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of ALOX5 gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers.

Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
As reported
Synthetic peptide corresponding to aa130-149 from human ALOX5 (KLH).
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, 0.5% BSA, 0.02% sodium azide, 50% glycerol.
Recognizes human ALOX5. Species sequence homology: Hamster, mouse, porcine and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.