Technical Data
A1359-76
ALOX5AP (Arachidonate 5-lipoxygenase-activating protein, FLAP, MK-886-binding protein)
Description:
This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:120,000
Western Blot: ~16kD
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide, C-HKVEHESRTQNGR, representing the internal region of the human protein according to NP_001620.2.
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human ALOX5AP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Kaaman M, Ryden M, Axelsson T, Nordstrom E, Sicard A, Bouloumie A, Langin D, Arner P, Dahlman I. ALOX5AP expression, but not gene haplotypes, is associated with obesity and insulin resistance.Int J Obes (Lond). 2006 Mar;30(3):447-52. PMID: 16261187