Technical Data
A1359-80
ALOX15 (Arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Peptide ELISA: 1:32,000.
Western Blot: 0.2-0.5µg/ml. Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.


Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See A1359-80-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
A synthetic peptide from the C-terminus of aa-HYKTDVAVKDDPE.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human ALOX15.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
McDuffie M, Maybee NA, Keller SR, Stevens BK, Garmey JC, Morris MA, Kropf E, Rival C, Ma K, Carter JD, Tersey SA, Nunemaker CS, Nadler JL.
Nonobese diabetic (NOD) mice congenic for a targeted deletion of 12/15-lipoxygenase are protected from autoimmune diabetes.
Diabetes. 2008 Jan;57(1):199-208. Epub 2007 Oct 16.
PMID: 17940120