Technical Data
A1359-80C
ALOX15 (Arachidonate 15-lipoxygenase, 15-Lipoxygenase 1, 15-Lipoxygenase Reticulocyte Arachidonate, 15-LOX, Arachidonate omega-6 Lipoxygenase)
Description:
ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1-5ug/ml using recombinant protein. The detection limit in sandwich ELISA is ~3ng/ml.
Immunohistochemistry (Paraffin): 1.5ug/ml stained human small intestine tissue.
Western Blot: 1-5ug/ml. Band detected at ~74.67kD using HeLa NE cell lysate. Band detected at ~98.93kD using immunogen protein lysate.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k3D8Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
ALOX15 (AAH29032, 1-663aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Purity:
Purified.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes human ALOX15.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Selective survival rescue in 15-lipoxygenase-1 deficient retinal pigment epithelial cells by the novel docosahexaenoic acid-derived mediator, neuroprotectin D1. Calandria JM, Marcheselli VL, Mukherjee PK, Uddin J, Winkler JW, Petasis NA, Bazan NG.J Biol Chem. 2009 Jun 26;284(26):17877-82. Epub 2009 Apr 29.