		Technical Data
A1359-80C	ALOX15 (Arachidonate 15-lipoxygenase, 15-Lipoxygenase 1, 15-Lipoxygenase Reticulocyte Arachidonate, 15-LOX, Arachidonate omega-6 Lipoxygenase)

Description:
ALOX15 converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. The protein also acts on C-12 of arachidonate as well as on linoleic acid.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1-5ug/ml using recombinant protein. The detection limit in sandwich ELISA is ~3ng/ml.
Immunohistochemistry (Paraffin): 1.5ug/ml stained human small intestine tissue.
Western Blot: 1-5ug/ml. Band detected at ~74.67kD using HeLa NE cell lysate. Band detected at ~98.93kD using immunogen protein lysate.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a,k	3D8	Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

ALOX15 (AAH29032, 1-663aa) full length recombinant protein with GST tag. MW of the GST tag alone is 26kD.

Purity:

Purified.

Form

Supplied as a liquid in PBS, pH 7.2.

Specificity:

Recognizes human ALOX15.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Selective survival rescue in 15-lipoxygenase-1 deficient retinal pigment epithelial cells by the novel docosahexaenoic acid-derived mediator, neuroprotectin D1. Calandria JM, Marcheselli VL, Mukherjee PK, Uddin J, Winkler JW, Petasis NA, Bazan NG.J Biol Chem. 2009 Jun 26;284(26):17877-82. Epub 2009 Apr 29.