Technical Data
A1372-02A
Alveolar Soft Part Sarcoma Chromosome Region, Candidate 1 (ASPSCR1, ASPCR1, ASPL, ASPS, RCC17, TUG)
Description:
The TUG protein contains a UBX domain, for GLUT4. In truncated form, TUG acts in a dominant-negative manner to inhibit insulin-stimulated GLUT4 redistribution in Chinese hamster ovary cells and 3T3-L1 adipocytes. Full-length TUG forms a complex specifically with GLUT4. In 3T3-L1 adipocytes, this complex is present in unstimulated cells and is largely disassembled by insulin. Endogenous TUG is localized with the insulin-mobilizable pool of GLUT4 in unstimulated 3T3-L1 adipocytes, and is not mobilized to the plasma membrane by insulin.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000
ELISA: 1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
Mab8A265Purified
SizeStorageShippingSourceHost
40ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Ni-NTA purified truncated recombinant TUG expressed in E. coli strain BL21 (DE3)
Purity:
Purified immunoglobulin
Form
Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.
Specificity:
Recognizes human Alveolar Soft Part Sarcoma Chromosome Region, Candidate 1 (ASPSCR1, ASPCR1, ASPL, ASPS, RCC17, TUG).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Jonathan S.B, Natalie H, Adrienne E. Lodish Functional cloning of TUG as a regulator of GLUT4 glucose transporter trafficking. Nature 2003,425:727 - 733.