		Technical Data
A1372-02A	Alveolar Soft Part Sarcoma Chromosome Region, Candidate 1 (ASPSCR1, ASPCR1, ASPL, ASPS, RCC17, TUG)

Description:
The TUG protein contains a UBX domain, for GLUT4. In truncated form, TUG acts in a dominant-negative manner to inhibit insulin-stimulated GLUT4 redistribution in Chinese hamster ovary cells and 3T3-L1 adipocytes. Full-length TUG forms a complex specifically with GLUT4. In 3T3-L1 adipocytes, this complex is present in unstimulated cells and is largely disassembled by insulin. Endogenous TUG is localized with the insulin-mobilizable pool of GLUT4 in unstimulated 3T3-L1 adipocytes, and is not mobilized to the plasma membrane by insulin.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:2000
ELISA: 1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab		8A265	Purified

Size		Storage	Shipping	Source	Host
40ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Ni-NTA purified truncated recombinant TUG expressed in E. coli strain BL21 (DE3)

Purity:

Purified immunoglobulin

Form

Supplied as a liquid in PBS, pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.

Specificity:

Recognizes human Alveolar Soft Part Sarcoma Chromosome Region, Candidate 1 (ASPSCR1, ASPCR1, ASPL, ASPS, RCC17, TUG).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Jonathan S.B, Natalie H, Adrienne E. Lodish Functional cloning of TUG as a regulator of GLUT4 glucose transporter trafficking. Nature 2003,425:727 - 733.