Technical Data
A1372-19
Alzheimer Precursor Protein A4
Description:
Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged humand and cases of Alzheimer's Disease. The principle component of this extracellular amyloid is beta/A4, a 4 kDa peptide derived from a larger amyloid precursor protein (APP), which is widely expressed in the brain and body. The creation, transport and function of these proteins are currently under investigation.

Applications:
Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: 5-10ug/ml on 4% paraformaldehyde/15% picric acid perfused and fixed rat spinal cord. Also good for paraffin-embeddedd tissues at 1:10- 1:20.
Western Blot: 10ug/ml under reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG12Q12Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein (66-81aa). Epitope: N-terminus.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes a N-terminal epitope on the human pre-A4 molecule. Species Crossreactivity: canine, fish, rat, mouse, monkey and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cell (1989) 57:115–126. 2. PNAS.USA (1990) 87:1561–1565. 3. PNAS.USA (1991) 88:1461–1465. 4. Brain Research (1983) 266:97–119. 5. Gasparini, L., et al., J. Neuroscience (2001) 21:2561–2570. 6. Mudher, A., et al., J. Neuroscience (2001) 21:4987–4995.