Technical Data
Media
Ames Medium w/L-Glutamine w/o Calcium Chloride, Magnesium Sulfate (Powder)
A1372-25A
Cell Culture Grade
Storage 4°C    Shipping RT
Components shown as g/liter
Inorganic Salts:
Potassium Chloride0.231
Potassium Phosphate Mono.0.068
Sodium Chloride7.01
Amino Acids:
L-Alanine0.0024
L-Arginine•HCl0.00421
L-Asparagine0.00084
L-Aspartic Acid0.00012
L-Cystine•2HCl0.000065
L-Glutamic Acid, Sodium0.001183
L-Glutamine0.073
Glycine0.00045
L-Histidine•HCl•H2O0.002513
L-Isoleucine0.00058
L-Leucine0.00144
L-Lysine•HCl0.003648
L-Methionine0.00039
L-Phenylalanine0.00132
L-Proline 0.00007
L-Serine0.00252
Taurine0.00075
L-Threonine0.00333
L-Tryptophan0.00049
L-Tyrosine•2Na•2H2O0.00211
L-Valine0.00176
Vitamins:
Ascorbic Acid•Na0.01796
D-Biotin0.0001
Choline Chloride0.0007
Folic Acid 0.0001
myo-Inositol0.0272
Niacinamide0.0001
D-Pantothenic Acid•Ca0.0001
Pyridoxal•HCl0.0001
Riboflavin0.00001
Thiamine•HCl0.0001
Other:
Cytidine0.00073
Thymidine0.00024
D-Glucose1.081
Hypoxanthine0.00082
Pyruvic Acid, Sodium0.01333
Uridine0.00073
Total:8.5g/liter
The retina is an important in vitro model for the central nervous system. It is more readily accessible than most nervous tissue and is strong enough to remain intact during manipulations. The retina of the rabbit commonly contains no penetrating blood vessels. It is nurtured by diffusion from capillary networks on either side. For this reason, the tissue will survive and functions without circulating blood, as long as it is bathed in a medium that closely resembles in vivo conditions.

Ames' medium was formulated to support retinal tissue in relatively short-term culture. Rabbit retina has been incubated in Ames' medium for over 2 days with its metabolism and electrical responses to light stimuli well maintained. This mixture is a medium of choice for maintaining central nervous system tissue in vitro.


Directions per Liter: Gas ~900ml of water (15-20°C) with 100% CO2. While gently stirring the water, add 1.9g sodium bicarbonate. Add 8.5g of the powder medium and stir until dissolved. Adjust the pH of the medium to 0.2-0.3 pH unit below the desired level using 1N NaOH and 1N HCl. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at 4°C. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to pale yellow, homogeneous, free flowing powder

Solubility (8.5g/L): Colorless to pale yellow, clear, complete

pH: As reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Ames, A., Nesbett, F., J. Neurochem. 37: 837 (1981).