Technical Data
A1372-31H2
AMICA (Adhesion Molecule that Interacts with CXADR Antigen 1, Junction Adhesion Molecule-Like, JAM-L, JAML) (Carboxyfluorescein)
Description:
AMICA (adhesion molecule, interacting with CXADR antigen 1), also known as JAML, is a 65kD, heavily glycosylated transmembrane protein that belongs to the junctional adhesion molecule (JAM) subset of the immunoglobulin superfamily (1). JAM family molecules contribute to intercellular connections within epithelial and endothelial cell layers, and mediate their interactions with various hemopoietic cells (1). The human AMICA cDNA encodes a 384 amino acid (aa) precursor that includes a 19 aa signal sequence, a 256 aa extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 98 aa cytoplasmic domain (2). Alternative splicing may generate isoforms with N- and C-terminal deletions. In contrast to other JAM family proteins, AMICA does not contain a cytoplasmic PDZ-binding motif (3). Within the ECD, human AMICA shares 58% and 63% aa sequence identity with mouse and rat AMICA, respectively. It shares 18%-20% aa sequence identity with the ECDs of human JAM-A, -B, -C, and JAM4. AMICA is expressed on the surface of granulocytes and monocytes and is up- regulated during the differentiation of myeloid leukemia cells (2, 3). A motif in the ECD, which promotes dimerization of other JAM family proteins, is required for surface localization of AMICA (2). AMICA mediates the adhesion of monocytes to endothelial cells (2) and neutrophil migration across epithelial cell monolayers (3). This latter function involves specific interactions of AMICA with coxsackie virus and adenovirus receptor (CXADR) in epithelial tight junctions (3). In particular, the membrane proximal Ig-like domain of AMICA binds the membrane-distal Ig-like domain of CXADR (3). AMICA does not appear to interact homophilically, as neutrophils adhere to immobilized CXADR but not to immobilized AMICA (3).

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a8H172Affinity Purified
SizeStorageShippingSourceHost
100Tests-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
NS0-derived rhAMICA (aa10-265)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with Carboxyfluorescein.
Specificity:
Recognizes human AMICA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mandell, K.J. and Parkos, C.A., 2005, Adv. Drug Deliv. Rev. 57:857. 2. Moog-Lutz, C. et al., 2003, Blood 102:3371. 3. Zen, K. et al., 2005, Mol. Biol. Cell 16:2694.