Technical Data
A1372-31H3
AMICA (Adhesion Molecule that Interacts with CXADR Antigen 1, Junction Adhesion Molecule-Like, JAM-L, JAML) (PE)
Description:
AMICA is a 65kD, heavily glycosylated transmembrane protein that belongs to the junctional adhesion molecule (JAM) subset of the immunoglobulin superfamily. JAM family molecules contribute to intercellular connections within epithelial and endothelial cell layers, and mediate their interactions with various hemopoietic cells. The human AMICA cDNA encodes a 384 amino acid (aa) precursor that includes a 19 aa signal sequence, a 256aa extracellular domain (ECD) with two Ig-like domains, a 21aa transmembrane segment, and a 98aa cytoplasmic domain. Alternative splicing may generate isoforms with N- and C-terminal deletions. In contrast to other JAM family proteins, AMICA does not contain a cytoplasmic PDZ-binding motif. Within the ECD, human AMICA shares 58% and 63% aa sequence identity with mouse and rat AMICA, respectively. It shares 18%-20% aa sequence identity with the ECDs of human JAM-A, -B, -C and JAM4. AMICA is expressed on the surface of granulocytes and monocytes and is up-regulated during the differentiation of myeloid leukemia cells. A motif in the ECD, which promotes dimerization of other JAM family proteins, is required for surface localization of AMICA. AMICA mediates the adhesion of monocytes to endothelial cells and neutrophil migration across epithelial cell monolayers. This latter function involves specific interactions of AMICA with coxsackie virus and adenovirus receptor (CXADR) in epithelial tight junctions. In particular, the membrane proximal Ig-like domain of AMICA binds the membrane-distal Ig-like domain of CXADR. AMICA does not appear to interact homophilically, as neutrophils adhere to immobilized CXADR but not to immobilized AMICA.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 1x10e5 cells or 50ul of packed whole blood.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Stable for at least 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a8H172Affinity Purified
SizeStorageShippingSourceHost
100Tests4C Do not freezeBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant corresponding to aa10-265 of human AMICA expressed in NS0 cells (Q86YT9).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).
Specificity:
Recognizes human AMICA. Does not crossreact with mouse AMICA. Species Sequence Homology: mouse (62%), canine (76%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Mandell, K.J. and Parkos, C.A., 2005, Adv. Drug Deliv. Rev. 57:857. 2. Moog-Lutz, C. et al., 2003, Blood 102:3371. 3. Zen, K. et al., 2005, Mol. Biol. Cell 16:2694.