Technical Data
A1372-32B
AIFM1 (Programmed Cell Death Protein 8, Apoptosis-inducing Factor 1, Mitochondrial, AIF, PDCD8)
Description:
Apoptosis-inducing factor (AIF) is a phylogenetically ancient mitochondrial intermembrane flavoprotein (reviewed in 1 and 2). Recombinant AIF induces caspase-independent peripheral chromatin condensation and large-scale DNA fragmentation when added to purified nuclei. Upon apoptosis induction, AIF translocates to the cytosol and the nucleus.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa593-606 of human AIF. (QLDLKTFEFNKELRYL); Genbank accession no. NP_004199.1.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human AIF. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hong SJ, TM Dawson and VL Dawson. 2004. Nuclear and mitochondrial conversations in cell death: PARP-1 and AIF signaling. Trends Pharmacol Sci. 25:259-264. 2. Cregan SP, VL Dawson, and RS Slack. 2004. Role of AIF in caspase-dependent and caspase-independent cell death. Oncogene. 23:2785-96.