Technical Data
A1372-32D
AIFM1 (Programmed Cell Death Protein 8, Apoptosis-inducing Factor 1, Mitochondrial, AIF, PDCD8)
Description:
Apoptosis-inducing factor (AIF) is a phylogenetically ancient mitochondrial intermembrane flavoprotein. AIF induces caspase-independent peripheral chromatin condensation and large-scale DNA fragmentation when added to purified nuclei. Upon apoptosis induction AIF translocates to the cytosol and the nucleus.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml
Immunohistochemistry (paraffin): 0.25-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human, mouse, rat heart lysate, or K562 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to human AIF.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human AIF. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Marzo I. et al. FEBS Lett 427(2):198-202 (1998). 2. Susin S.A., et al. Nature. Feb 4;397(6718):441-6 (1999). 3. Cande C., et al. Biochimie 84(2-3):215-222 (2002).