Technical Data
A1372-37
AMICA (Adhesion Molecule that Interacts with CXADR Antigen 1, Junction Adhesion Molecule-Like, JAM-L, JAML)
Description:
AMICA (Adhesion Molecule that interacts with CXADR antigen 1), also known as junction adhesion molecule like (JAM-L) is a 60kD type I transmembrane glycoprotein member of the JAM family of adhesion molecules. Human AMICA has an extracellular domain (ECD) of 275 amino acids (aa) and a cytoplasmic tail of 98 amino acids. The ECD contains two Ig-like regions. There is an N-terminal C2-type Ig-like domain and a membrane proximal V-type Ig-like domain. AMICA is expressed by leukocytes and is concentrated at areas of cell-cell contact. It forms noncovalent homodimers on the cell surface and binds CXADR on epithelial cells. Two splice variants apparently exist that only involve the first 40 aa of the precursor. Human AMICA ECD is 62% and 76% aa identical to mouse and dog AMICA ECD, respectively.

Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b7H215Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
As reported
Immunogen:
NS0-derived recombinant human AMICA (aa10-265) Accession # Q86YT9
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile ddH2O or sterile 40-50% glycerol.
Specificity:
Recognizes human AMICA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.