Technical Data
AMICA (Adhesion Molecule that Interacts with CXADR Antigen 1, Junction Adhesion Molecule-Like, JAM-L, JAML)
AMICA (Adhesion Molecule that interacts with CXADR antigen 1), also known as junction adhesion molecule like (JAM-L) is a 60kD type I transmembrane glycoprotein member of the JAM family of adhesion molecules. Human AMICA has an extracellular domain (ECD) of 275 amino acids (aa) and a cytoplasmic tail of 98aa. The ECD contains two Ig-like regions. There is an N-terminal C2-type Ig-like domain and a membrane proximal V-type Ig-like domain. AMICA is expressed by leukocytes and is concentrated at areas of cell-cell contact. It forms noncovalent homodimers on the cell surface and binds CXADR on epithelial cells. Two splice variants apparently exist that only involve the first 40aa of the precursor. Human AMICA ECD is 62% and 76% aa identical to mouse and canine AMICA ECD, respectively.

Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml. Using a colormetrc detection system the detection limit for recombinat human AMICA is 1ng/lane under non-reducing and reducing conditions.
ELISA: 0.5-1ug/ml. The detection limit for recombinat human AMICA is ~3ng/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2b7H215Affinity Purified
100ug-20CBlue IceHumanMouse
~0.5mg/ml (after reconstitution)
Recombinant protein corresponding to aa10-265 of human AMICA (Q86YT9), expressed in NSO cells.
Purified by Protein G affinity chromatography.
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Recognizes human AMICA. Does not react with recombinant mouse AMICA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.