Technical Data
Media
Amies Broth with Charcoal (Amies Transport Medium) (Powder)
A1372-40
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Calcium Chloride 0.1
Charcoal10.0
Magnesium Chloride 0.1
Potassium Chloride 0.2
Monopotassium Phosphate 2.5
Sodium Chloride 3.0
Disodium Phosphate 1.15
Sodium Thioglycollate 1.0
Total:18.05g/liter
Amies fomulated a medium for transport of microorganisms using a balanced salt solution. Amies Medium with Charcoal is a non-nutritional, phosphate buffered type medium used to maintian the viablilty of microorganisms without a significant increase in growth.

Description:
Amies formulated a medium for transport of microorganisms using a balanced salt solution. Amies Broth with Charcoal is a non-nutritional, phosphate buffered type medium used to maintain the viability of microorganisms without a significant increase in growth. Charcoal was added to the formulation to neutralize the toxic effects of a fatty acids that are toxic to microorganisms. This medium is recommended for throat, vaginal, and wound samples.


Directions per Liter: Dissolve 18.05 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Black, homogeneous, free flowing powder Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.