Technical Data
Amies Medium w/o Charcoal (Amies Transport Medium) (Powder)
Microbiological Grade
Storage RT    Shipping RT
Components shown as g/liter:
Calcium Chloride0.1
Magnesium Chloride0.1
Potassium Chloride0.2
Potassium Phosphate Mono2.5
Sodium Chloride3
Sodium Phosphate Dibasic1.15
Sodium Thioglycolate1
Amies fomulated a medium for transport of microorganisms using a balanced salt solution. Amies Medium without Charcoal is a non-nutritional, phosphate buffered type medium used to maintian the viablilty of microorganisms without a significant increase in growth.

Appearance: White, homogeneous, free flowing powder

Solubility: Colorless and opalescent, with precipitate.

pH: As Reported

Directions per Liter: Dissolve 8.05 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.