Technical Data
A1374-08R
ACY3, Recombinant, Human, aa1-319, His-Tag (Aspartoacylase-2, Aminoacylase-3, ACY-3, Acylase III, Hepatitis C Virus Core-binding Protein 1, HCBP1, ASPA2)
50ug
Molecular Biology Storage: -20CShipping: Blue Ice
Aspartoacylase 3, also known as ACY3, is member of the Aspartoacylase subfamily. It plays an important role in deacetylating mercapturic acids in kidney proximal tubules. ACY3 localizes to the cytoplasm of S2 and S3 proximal tubules and to the apical domain of S1 proximal tubules. ACY3 protein is also expressed at low levels in stomach, testis, heart, brain, lung and liver, and may function as an HCV (Hepatitis C virus) core binding protein. Purified by using conventional chromatography techniques.

Source:
Recombinant corresponding to aa1-319 of human ACY3, fused to His-tag at N-terminal, expressed in E.coli.

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSMCSLPVP REPLRRVAVT GGTHGNEMSG VYLARHWLHA PAELQRASFS AVPVLANPAA TSGCRRYVDH DLNRTFTSSF LNSRPTPDDP YEVTRARELN QLLGPKASGQ AFDFVLDLHN TTANMGTCLI AKSSHEVFAM HLCRHLQLQY PELSCQVFLY
QRSGEESYNL DSVAKNGLGL ELGPQPQGVL RADIFSRMRT LVATVLDFIE LFNQGTAFPA FEMEAYRPVG VVDFPRTEAG HLAGTVHPQL QDRDFQPLQP GAPIFQMFSG EDLLYEGEST VYPVFINEAA YYEKGVAFVQ TEKFTFTVPA MPALTPAPSP AS

Molecular Weight:
~37.6kD (342aa), confirmed by MALDI-TOF

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~37.6kD
Source: E.coli
Purity: ~90% (SDS-PAGE)
Concentration: ~0.5mg/ml
Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8, 1mM DTT, 0.1M sodium chloride, 10% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Chen Y R., et al. (2009) J Gastroenterol Hepatol. 24(7):1300-4. Pushkin A., et al. (2004). Am J Physiol Cell Physiol. 286(4):C848-56.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.