		Technical Data
A1379-50C	AAP-S, ID (Puromycin-sensitive Aminopeptidase, Cytosol Alanyl Aminopeptidase, PSA, NPEPPS)

Description:
PSA is the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Flow Cytometry: 1:10-1:50
Immunohistochemistry: 1:50-1:100
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

KLH-conjugated synthetic peptide located between aa543-573 in the center region of human PSA

Purity:

Purified by ammonium sulfate precipitation

Form

Supplied as a liquid in PBS, 0.09% sodium azide

Specificity:

Recognizes human PSA

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Thompson,M.W., Peptides 24(9), 1359-1365 (2003).