Technical Data
Ammonia BioAssay™ Kit
Kits and Assays Storage: 4°CShipping: Blue Ice
Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the
synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic
product which is created through amino acid deamination. It plays an important role in both
normal and abnormal animal physiology such as acid/base balance. USBio provides a rapid, simple, sensitive, and reliable assay suitable for research and high throughput assay of Ammonia. In this non-enzymatic assay, ammonia is used to form indophenol, a highly colored product easily quantifiable by colorimetry (OD670nm) using a plate reader. The kit can detect less than 1nmol (~10uM) ammonia using either a 96 well or 384 well microwell plate.

Kit Components:
Ammonia Reagent 1 1x8ml
Ammonia Reagent 2 1x4ml
Ammonium Chloride Standard (100mM) 1x0.1ml.

Storage and Stability:
Store at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
E.D. Rhine, G.K. Pratt, R.L. Mulvaney and E.J. Pratt (1998) Soil Sci. Soc. AM. J. 62: 473-480

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.