Technical Data
A1401
4-Amino Biphenyl DNA (4-AMBP DNA, 4-Aminobiphenyl DNA)
Description:
4-amino Biphenyl (4-ABP) is an aromatic amine (arylamine). 4-ABP can be found as a constituent of tobacco smoke, in the form of an environmental carcinogen. It is metabolized by hepatic cytochrome P4501A2 to yield a direct-acting mutagen known as N-hydroxy-aminobiphenyl. Yet, the accumulation of biologically active metabolites is dependent on the activation and detoxification of N-acetylation.

4-ABP has been shown to be a bladder carcinogen in humans and rodents. As well as genetic factors, 4-ABP may also play a role in breast cancer. It is hypothesized that smokers may have higher levels of DNA adducts than non-smokers and that the role of 4-ABP-DNA adducts and their relationship to tobacco exposure may be related to breast tumorigenesis. The antibody has been used to investigate the role of 4-ABP in the study of human breast cancer by measuring 4-ABP-DNA adducts.

Cellular Localization: Nuclear

Applications:
Suitable for use in Immunofluorescence, ELISA, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:50-1:100
Immunohistochemistry: 1:50-1:100
Immunocytochemistry: 1:50-1:100
Immunofluorescence: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
Mab8J250Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
4-amino Biphenyl DNA.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid (ascites).
Specificity:
This antibody is specific for 4-amino Biphenyl DNA. Crossreactivity: All DNAs
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. al-Atrash, J., et al, Quantitative immunohistochemical analysis of 4-aminobiphenyl DNA in cultured cells and mice: comparison to gas chromatography/mass spectroscopy analysis. Chem. Res. Toxicol. 8(5): 747-752(1995) [uses this clone] 2. McQueen, CA., et al, Neonatal ontogeny of murine arylamine N-acetyltransferases: implications for arylamine genotoxicity. Toxicol. Sci. 73: 279-286 (2003) [uses this clone] 3. Faraglia, B., et al, Evaluation of 4-aminobiphenyl-DNA adducts in human breast cancer: the influence of tobacco smoke. Carcinogenesis. 24(4): 719-725 (2003) [uses this clone] 4. Al-Atrash, J., et al. Quantitative immunohistochemical analysis of 4-aminobiphenyl DNA in cultured cells and mice: comparison to gas chromatography/mass spectroscopy analysis. Chem Res Toxicol. 8(5):747-52, 1995. 5. Brown, A., et al. Robust DNA Repair May Lower Breast Cancer Risk. Journal of the National Cancer Institute. 2005.