Technical Data
A1475-02P
AMP Activated Protein Kinase alpha1, Control Peptide (AMPK a1)
50ug
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Technical Data |
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A1475-02P |
AMP Activated Protein Kinase alpha1, Control Peptide (AMPK a1) |
50ug |
| Molecular Biology | Storage: -20°CShipping: Blue Ice |
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AMPK is responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. It appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. AMPK alpha1, a member of the Ser/Thr protein kinase family, is a catalytic subunit of AMPK. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: blocks the antibody activity completely by incubating the peptide with equal volume of antibody for 30-60 minutes at 37°C. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
Concentration: ~0.5mg/ml Form: Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.02% thimerosal, 50% glycerol. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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