		Technical Data
A1580	AMPH (AMPH1, amphiphysin (Stiff-Man syndrome with breast cancer 128kD autoantigen), HGNC:471)

Description:
Amphiphysin I is a 76kD protein with an apparent molecular mass of 128kD, that associates with the cytoplasmic surface of synaptic vesicle (1). Amphiphysin is highly enriched in nerve terminal, where it has a putative role in clathrin-dependent recycling of secretory vesicle (2). Amphiphysin binds dynamin 1 and synaptojanin 1 through carboxy terminal SH3 domains (3). Additional interactions have been observed with clathrin and AP-2. It is through these interactions that amphiphysin is thought to play a role in clathrin mediated endocytosis. Amphiphysin is the dominant autoantigen in paraneoplastic Stiff-Man syndrome (4).

Applications:
Western Blot (Colorimetric): 0.5ug/ml
Immunoprecipitation
Immunohistochemistry: 5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Controls:
Rat Brain Tissue Extract

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG	4E8	Affinity Purified

Size		Storage	Shipping	Source	Host
25ug		-20°C	Blue Ice	Human	Mouse

Concentration:

As reported

Immunogen:

Recombinant human amphiphysin

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.1mM PMSF.

Specificity:

Detects a 128kD protein, corresponding to the apparent molecular mass of amphiphysin I on SDS-PAGE immunoblots, in samples from human, monkey, rat, mouse, rabbit and bovine. A 108kD isoform may be observed in some tissues or cell lysates. The epitope is located within the carboxyl terminal region of amphiphysin I (residues 545–695). Rat brain immunostaining using this antibody in immunofluorescence shows synaptic distribution. Species Reactivity: human, monkey, rat, mouse, rabbit, bovine

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Floyd, S., Butler, M.H., Cremona, O., David, D., Freyberg, Z., Zhang, X., Solimena, M., Tokunaga, A., Ishizu, H., Tsutsui, K. and De Camilli, P. (1998) Mol. Med. 4: 29–39. 2. Slepnev, V.I., Ochoa, G.C., Butler, M.H., Grabs, D. and De Camilli, P.D. (1998) Science 281: 821–824. 3. David, C., McPherson, P.S., Mundigl, O. and De Camilli, P. (1996) Proc. Natl. Acad. Sci. USA 93: 331–335. 4. Yamammoto, R., Li, X., Winter, S., Francke, U., Kilimana, M.W. (1995) Hum. Mol. Genet. 4: 265–268.