Technical Data
AMPH (AMPH1, Amphiphysin)
AMPH is a member of the Amphiphysin family, BAR superfamily of proteins. Although its calculated MW is only 78kD, it runs anomalously in SDS-PAGE at 125-128kD. It is expressed in neurons, Sertoli cells, possibly in anterior pituitary endocrine cells and apparently cardiac muscle. It may form heterodimers with AMPH2, and in this configuration, promotes clathrin-associated endocytic vesicle formation. Furthermore, and perhaps related, AMPH also interacts with N-WASP, initiating actin polymerization. Human AMPH is 695aa in length. It contains an N-terminal BAR domain aa24-240 that binds lipids, a central region that binds AP2 aa322-340 and clathrin aa347-386, and a C-terminal SH3 domain aa622-695 that binds proline-rich ligands. There is one known 108kD splice variant that shows a deletion of aa425-466. Over aa204-300, human AMPH shares 98% aa identity with mouse AMPH.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2B12K8Affinity Purified
100ug-20CBlue IceHumanMouse
~0.5mg/ml after reconstitution
Recombinant corresponding to aa204-300 from human AMPH expressed in E.coli.
Purified by Protein A/G affinity chromatography.
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Recognizes human Amphiphysin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.