Technical Data
A1585
Amphiregulin (Schwannoma-derived Growth Factor, Amphiregulin precursor, AR, Colorectum cell-derived growth factor, CRDGF, MGC13647, SDGF)
Description:
Amphiregulin (AR) which binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84aa residue glycosylated polypeptide growth regulator. It is generated by proteolytic processing of a 252aa transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes. These ligands are capable of binding to the extracellular domain of EGFR. They include EGF, TGFa, AR, HB-EGF, cripto-1, epiregulin and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein is localized to the epithelium of the colon, stomach, pancreas, breast and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach and pancreas.

Applications:
Suitable for use in ELISA and Immunoprecipitation. Other applications not tested

Recommended Dilution:
Immunoprecipitation (Native verified): 10ug/mg protein lysate. Use Protein A.
Optimal dilutions to be determined by the researcher.

Positive Control:
LS174T cells

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide, corresponding to aa8-26 (VKPPQNKTESENTSDKPKR) from the secreted amphiregulin of human origin. MW: 18.5-22.5kD. Cellular Localization: Cytoplasmic and/or nuclear.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, 0.2% BSA. Also supplied without BSA and sodium azide. See cat# A1585X.
Specificity:
Highly specific to AR. Does not crossreact with EGF, TGFa or HB-EGF. Species Crossreactivity: Human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Johnson, G.R., et al., J. Cell. Biol. 118: 741-751 (1992). 2. Johnson, G.R., et al., J. Biol. Chem. 268: 18835-18843 (1993). 3. Johnson, G.R., Wong, L., J. Biol. Chem. 269: 27149-27154 (1994). 4. Martinez-Lacaci, I., et al., Endocrinology 136: 3983-3992 (1995). 5. Schuger, L., et al. Development