Technical Data
A1585-01A
Amphiregulin (Schwannoma-derived Growth Factor, Amphiregulin precursor, AR, Colorectum cell-derived growth factor, CRDGF, MGC13647, SDGF)
Description:
Amphiregulin (AR) binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84-amino acid residue glycosylated polypeptide growth regulator, which is generated by proteolytic processing of a 252-amino acid transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes, are capable of binding to the extracellular domain of EGFR. These ligands include EGF, TGFalpha, AR, HB-EGF, cripto-1, epiregulin, and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein has been localized by immunohistochemistry to the epithelium of the colon, stomach, pancreas, breast, and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach, and pancreas.

Cellular Localization: Cytoplasmic and/or nuclear

Positive Control: LS174T cells

Applications:
Suitable for use in Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic 19 mer peptide, corresponding to amino acids 8 - 26 (VKPPQNKTESENTSDKPKR) from the secreted amphiregulin of human origin.
Purity:
Purified by Protein A affinity chromatography.
Form
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
Specificity:
Species Crossreactivity: Crossreacts with Human and Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Johnson G R, et al. (1992) J Cell Biol 118:741-751 2. Johnson G R, et al. (1993) J Biol Chem 268:18835-18843 3. Johnson G R and Wong L. (1994) J Biol Chem 269:27149-27154 4. Martinez-Lacaci I et al. (1995) Endocrinology, 136:3983-3992 5. Lucia Schuger; et al. (1996) Development, 122:1759-1767.