Technical Data
A1585-01B
Amphiregulin (Schwannoma-derived Growth Factor, Amphiregulin precursor, AR, Colorectum cell-derived growth factor, CRDGF, MGC13647, SDGF, AREG)
Description:
Amphiregulin (AR) which binds to EGF-Receptor (EGFR) with lower affinity than EGF. The mature secreted form AR is an 84aa residue glycosylated polypeptide growth regulator. It is generated by proteolytic processing of a 252aa transmembrane precursor. Seven different polypeptide ligands, which derive from distinct genes. These ligands are capable of binding to the extracellular domain of EGFR. They include EGF, TGFa, AR, HB-EGF, cripto-1, epiregulin and betacellulin. All of these growth factors contain a characteristic EGF-like domain which is defined by 6 evenly spaced cysteine residues that generate 3 loops through the formation of disulfide bonds. AR protein is localized to the epithelium of the colon, stomach, pancreas, breast and placenta. AR is reportedly overexpressed in human cancers of breast, colon, stomach and pancreas.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~0.1mg/ml
Immunogen:
Synthetic peptide corresponding to aa8-26 from human AREG (KLH).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% BSA, 0.05% sodium azide.
Specificity:
Recognizes human AREG. Species sequence homology: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.