Technical Data
Ampicillin Beta-Lactamase Modified, 1000X
Biochemicals Storage: -20CShipping: Blue Ice
Ampicillin Beta-Lactamase Modified, is a unique antibiotic that is a stronger inhibitor of b-Lactamase than ampicillin, carbenicillin or methicillin. Ampicillin is modified utilizing a novel method that results in improved transformation efficiencies where higher cell density and longer incubation times traditionally result in the development of ampicillin sensitive satellite colonies. When used at a concentration of 1X, the presence of satellite colonies Identificatis reduced, even after prolonged incubation (2-4 days) and plating of competent cells at 20 fold higher density.

No background (satellite) colonies are observed
Allows higher density plating
Longer incubation times are readily achieved at 37C
Supplied as a convenient 1000X solution

Quality Control:
E. coli DH5a cells were transformed with pUC19 DNA and plated on LB agar plates containing 150g ampicillin and 1X Ampicillin Beta-Lactamase Modified and incubated at 37C for 24 hours. The plate containing ampicillin had greater than 103 satellite colonies while the plate containing Ampicillin, Beta-Lactamase Modified showed no satellite colonies.

Ampicillin Beta-Lactamase Modified 1000X , 3 x 1ml.

Instruction for Use:
b-Lactamase Modified Ampicillin is supplied as a ready solution at a concentration of 1000X.
To use, add 1ml per liter of the medium. Do not add to the medium at temperatures above 60C.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.