Technical Data
Ampicillin Dextrin Agar (Powder)
Microbiological Grade
Storage RT/4°C    Shipping RT
Components shown as g/Liter
Bromothymol Blue0.04
Ferric Chloride•6H2O0.1
Magnesium Sulfate•7H2O0.2
Potassium Chloride2
Sodium Chloride3
Yeast Extract2
500g makes13.4 Liters
Media used for enumeration of Aeromonas species in water by membrane filtration.

Directions per Liter: Suspend 37.3 grams in liter of distilled or deionized water. Stir to mix thoroughly. Heat to boiling to dissolve completely. Autoclave for 15 minutes at 121ºC (15psi). Cool to 45-50°C. Add 10mg of sterile Ampicillin to inhibit E. faecalis. Alternatively, an additional 2mg sterile Vancomycin may be added to create Ampicillin Dextrin Agar with Vancomycin (ADA-V) to enumerate Aeromonas in finished waters. If detecting Aeromonas spp. in sea water, 50mg of the vibriostatic agent O129 can be added to the ADA medium.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Beige, homogenous, free flowing powder

Solubility: Green, clear, complete after autoclaving pH

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.