Technical Data
Media
Ampicillin Dextrin Agar (Powder)
A2259-50
Microbiological Grade
Storage RT/4°C    Shipping RT
Components shown as g/Liter
Agar15
Bromothymol Blue0.04
Dextrin10
Ferric Chloride•6H2O0.1
Magnesium Sulfate•7H2O0.2
Potassium Chloride2
Sodium Chloride3
Tryptose5
Yeast Extract2
Total:37.3g/Liter
500g makes13.4 Liters
Media used for enumeration of Aeromonas species in water by membrane filtration.


Directions per Liter: Suspend 37.3 grams in liter of distilled or deionized water. Stir to mix thoroughly. Heat to boiling to dissolve completely. Autoclave for 15 minutes at 121ºC (15psi). Cool to 45-50°C. Add 10mg of sterile Ampicillin to inhibit E. faecalis. Alternatively, an additional 2mg sterile Vancomycin may be added to create Ampicillin Dextrin Agar with Vancomycin (ADA-V) to enumerate Aeromonas in finished waters. If detecting Aeromonas spp. in sea water, 50mg of the vibriostatic agent O129 can be added to the ADA medium.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Beige, homogenous, free flowing powder

Solubility: Green, clear, complete after autoclaving pH


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.