Technical Data
Amylin, Rat, Control Peptide (DAP, Diabetes-Associated Peptide, IAP, Islet Amyloid Polypeptide, Insulinoma Amyloid Peptide, IAPP)
Molecular Biology Storage: -20CShipping: Blue Ice
Control peptide for A2275-53P.

The calcitonin family of bioactive peptides comprises of calcitonin, amylin, two calcitonin-gene related peptides (CGRP1, and CGRP2) and adrenomedullin (ADM). Amylin is a 37aa peptide produced in the pancreatic beta-cell secretory granules and is co-released with insulin. Amylin also has CGRP-like effects on bone metabolism. Amylin has specific binding sites in the CNS and it may regulate gastric emptying and influence carbohydrate metabolism. The calcitonin family peptides probably act through G-protein coupled membrane receptors. Recently, a homolog of calcitonin receptor, CRLR (calcitonin-receptor-like receptor human 461aa; rat/mouse 463aa) was identified. It is now shown that CRLR can function as either a CGRP receptor or an ADM receptor, depending upon which members of a new family of proteins called receptor activity modifying proteins (RAMP1-3) are expressed.

Synthetic peptide corresponding to 13aa from the ~mid-region of mature of Amylin 18-30aa of rat AMYL/AML (accession #P12969).

Molecular Weight:

Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 5-10ug control peptide per 1 g of aff pure IgG or 1ul antiserum
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Synthetic peptide
Purity: Purified
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Mosselman S et al (1989) FEBS lett. 247, 154. 2. Nishi M et al (1989) Mol. Endocrinol. 3, 177. 3. Sanke T et al (19880 JBC 263, 17243. 4. Christmanson L et al (1990) FEBS lett. 267, 160. 5. westmark P et al (1987) ONAS 86, 9662. 6. McLatchie LM et al (1998) Nature 393, 333-339. 7. Nagae T et al (2000) BBRC 270, 89-93. 8. Husmann, K et al (2000). Mol Cell Endocrinol (2000) 162, 35-43.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.