		Technical Data
A2275-61Q	APBB1, phosphorylated (Ser175) (Amyloid beta A4 Precursor Protein-binding Family B Member 1, Fe65 Protein, FE65, RIR)

Description:
APBB1 is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser175 of human APBB1 (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human APBB1 when phosphorylated at Ser175.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Kinoshita, A., et al., J. Biol. Chem. 278(42):41182-41188 (2003). 2. Walsh, D.M., et al., Biochemistry 42(22):6664-6673 (2003). 3. Zhao, Q., et al., Biochemistry 42(12):3627-3634 (2003). 4. Hu, Q., et al., Hum. Mol. Genet. 11(4):465-475 (2002). 5. Bruni, P., et al., J. Biol. Chem. 277(38):35481-35488 (2002).