Technical Data
A2275-69L
Amyloid Precursor Protein, Human (APP) BioAssay™ ELISA Kit
96Tests
Kits and Assays Storage: 4°C/-70°CShipping: Blue Ice
Sensitivity:
<0.4ng/ml The analytical sensitivity of this assay is <0.4ng/ml of Human APP. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 30 times.

Specificity:
The Human APP BioAssay™ ELISA kit is specific for measurement of total APP protein. The following proteins were tested at 100ng/ml and found to have no crossreactivity: Tau, a-synuclein, b-synuclein, Ab1-40 and Ab1-42.

Range: 0.78-50ng/ml

Incubation Time:
4 hours

Sample Size:
10ul (diluted to a total volume of 100ul)

Sample Type:
Cell lysates and cerebral spinal fluids

Specifications:
Simple: Pre-coated strip-well plates.
Liquid stable chromogen and stop reagents.
Economical: One 96-well plate, plus reagents for 96 determinations.
Reagents are stable for multiple runs.

Precision:
Intra-assay: <8%
Inter-assay: <9%

Kit Components:
*A2275-69L1: Standard, 2x1 vial
Supplied as a lyophilized powder. Reconstitute with 1ml of A2275-69L2: Standard Diluent Buffer.
A2275-69L2: Standard Diluent Buffer,1x25ml
Supplied as a liquid containing 8mM sodium azide.
A2275-69L3: Microtiter Plate, 1x96 wells
A2275-69L4: anti-APP (Biotin), 1x11ml
A2275-69L5: Streptavidin (HRP) (100X), 1x125ul
Supplied as a liquid containing 1.7mM thymol.
A2275-69L6: Streptavidin (HRP) Diluent, 1x25ml
Supplied as a liquid containing 1.7mM thymol and 0.05% Proclin 300.
A2275-69L7: Wash Buffer (25X), 1x100ml
A2275-69L8: TMB, 1x25ml
A2275-69L9: Stop Solution, 1x25ml

Storage and Stability:
Store *A2275-69L1: Standard powder at 4°C, liquid at -70°C. Store other components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Palmer, M.R., et al., Biochem. Biophys. Res. Comm. 156: 432-437 (1988). 2. Selkoe, D.J., et al., PNAS USA 85: 7341-7345 (1988). 3. Haniu, M., et al., J. Biol. Chem. 275: 21,099-21,106 (2000). 4. Vassar, R., et al., Science 286: 735-741 (1999). 5. Yan, R., et al., Nature 402: 533-537 (1999). 6. Pinnix, I., et al., FASEB J. 15: 1810-1812 (2001). 7. Lleo, A., et al., J. Biol. Chem. 2003 Sep. 5 [Epub ahead of print]. 8. Turner, P.R., et al., Prog. Neurobiol. 70: 1-32 (2003). 9. Sergeant, N., et al., J. Neurochem. 85: 1581-1591 (2003).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.