Amyloid Precursor Protein, Human (APP) BioAssay™ ELISA Kit
|Kits and Assays||Storage: 4°C/-70°CShipping: Blue Ice|
<0.4ng/ml The analytical sensitivity of this assay is <0.4ng/ml of Human APP. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 30 times.
The Human APP BioAssay™ ELISA kit is specific for measurement of total APP protein. The following proteins were tested at 100ng/ml and found to have no crossreactivity: Tau, a-synuclein, b-synuclein, Ab1-40 and Ab1-42.
10ul (diluted to a total volume of 100ul)
Cell lysates and cerebral spinal fluids
Simple: Pre-coated strip-well plates.
Liquid stable chromogen and stop reagents.
Economical: One 96-well plate, plus reagents for 96 determinations.
Reagents are stable for multiple runs.
*A2275-69L1: Standard, 2x1 vial
Supplied as a lyophilized powder. Reconstitute with 1ml of A2275-69L2: Standard Diluent Buffer.
A2275-69L2: Standard Diluent Buffer,1x25ml
Supplied as a liquid containing 8mM sodium azide.
A2275-69L3: Microtiter Plate, 1x96 wells
A2275-69L4: anti-APP (Biotin), 1x11ml
A2275-69L5: Streptavidin (HRP) (100X), 1x125ul
Supplied as a liquid containing 1.7mM thymol.
A2275-69L6: Streptavidin (HRP) Diluent, 1x25ml
Supplied as a liquid containing 1.7mM thymol and 0.05% Proclin 300.
A2275-69L7: Wash Buffer (25X), 1x100ml
A2275-69L8: TMB, 1x25ml
A2275-69L9: Stop Solution, 1x25ml
Storage and Stability:
Store *A2275-69L1: Standard powder at 4°C, liquid at -70°C. Store other components at 4°C. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.