Technical Data
A2275-71K1
APBA1 (Amyloid beta A4 Precursor Protein-binding Family A Member 1, Neuron-specific X11 Protein, Neuronal Munc18-1-interacting Protein 1, Mint-1, Adapter Protein X11alpha, X11, MINT1)
Description:
APBA1 is a member of the X11 protein family. It is a neuronal adapter protein that interacts with the Alzheimer's disease amyloid precursor protein (APP). It stabilizes APP and inhibits production of proteolytic APP fragments including the A beta peptide that is deposited in the brains of Alzheimer's disease patients. This protein is believed to be involved in signal transduction processes. It is also regarded as a putative vesicular trafficking protein in the brain that can form a complex with the potential to couple synaptic vesicle exocytosis to neuronal cell adhesion.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:16,000
Western Blot: 0.1-0.3ug/ml, observed in human brain, cerebellum and hippocampus, lysates on ~100kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-EASHPSQDGKRQYK, from human APBA1, at the internal region of the protein (NP_001154.2).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human APBA1. Species sequence homology: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1 .Jacobs EH, Williams RJ, Francis PT. Cyclin-dependent kinase 5, Munc18a and Munc18-interacting protein 1/X11alpha protein up-regulation in Alzheimer's disease. Neuroscience. 2006;138(2):511-22. Epub 2006 Jan 18.