Technical Data
A2275-71M
Amyloid Precursor Protein (APP, Amyloid beta (A4) Precursor Protein)
Description:
The C99 fragment itself is a substrate for gamma-secretase to generate the 4kD beta amyloid peptide which is found in the brains of Alzheimer's disease patients.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal working dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa737-751 of human APP. This sequence corresponds to aa85-99 of the C99 fragment.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide. No stabilizing proteins added.
Specificity:
Recognizes both human intact APP and the C99 fragment generated by Beta-secretase. Species Crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ponte, P. et al. (1988). A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 331: 525-527. 2. Selkoe, D.J. (1994). Cell biology of the amyloid beta protein precursor and the mechanism of Alzheimer's disease. Ann. Rev. Cell. Biol. 10: 373-403.