The C99 fragment itself is a substrate for gamma-secretase to generate the 4kD beta amyloid peptide which is found in the brains of Alzheimer's disease patients.
Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested.
Western Blot: 1:500-1:1000
Optimal working dilutions to be determined by researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Synthetic peptide corresponding to aa737-751 of human APP. This sequence corresponds to aa85-99 of the C99 fragment.|
|Purified by affinity chromatography.|
|Supplied as a liquid in PBS, 0.02% sodium azide. No stabilizing proteins added.|
|Recognizes both human intact APP and the C99 fragment generated by Beta-secretase. Species Crossreactivity: mouse, rat.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Ponte, P. et al. (1988). A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 331: 525-527. 2. Selkoe, D.J. (1994). Cell biology of the amyloid beta protein precursor and the mechanism of Alzheimer's disease. Ann. Rev. Cell. Biol. 10: 373-403.|