Technical Data
-Amyloid 1-42, Human (Control Peptide)
Molecular Biology Storage: -20CShipping: Blue Ice
Control Peptide for A2275-75S and A2275-75T. Human, A synthetic peptide consisting of 8aa of human beta 1-42 mapping within to the C-terminal. High degree of sequence homology with Mouse b-1-42. Demonstrates minimal sequence homology with b-1-40.

Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 aa peptides (beta amyloid1-40, 1-42, and 1-43). Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein. The 140 aa a-synucleins is identical with non-Ab component (NACP) of AD. The 134 aa human b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14. Mutations in a-synuclein gene causing a replacement of alanine with a threonine may cause the protein to misfold. Synucleins are primarily expressed in the brain. At least 3 forms: two large (140 aa SYN-1 & 149 aa SYN-2) and a small form (SYN-3, 42 aa) are produced by alternative splicing.

Suitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 50-100ng of control peptide/well.
Antibody Blocking: 5-10ug per 1ug A2275-75S (affinity purified antibody) or per 1ul A2275-75T (antiserum).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Human synthetic peptide
Purity: Highly purified
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS, pH 7.4.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Neve, R., et al., PNAS 89: 3448 (1992). 2. Goldgaber, D., et al., Science 235: 877 (1987). 3. Yankner, B., et al., Science 245: 417 (1989). 4. Golde, T., et al., Science 255: 728 (1992). 5. Wang, R., et al., J. Biol. Chem. 266: 16,960 (1991). 6. Shigematuse, K., et al., J. Neurosci. 31: 443 (1992). 7. Kang, J., et al., Nature 325: 733 (1987). 8. Tanzi, R., et al., Science 235: 880 (1987). 9. Weidmann, A., et al., Cell 57: 115 (1989).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.