Technical Data
A2275-78A
b-Amyloid
Description:
Amyloid beta-protein/amyloid A4 or amyloid beta-peptide is derived from the amyloid beta-precursor protein. Beta amyloid is an extracellular filamentous protein deposit found in the
brain. It is the major protein component of amyloid cores, neuritic plaques and is also found as a deposit in neurofibrillary tangles. In man, Alzheimer’s disease is the most common cause of senile dementia and is characterized by abnormal filamentous protein deposits in the brain. Beta amyloid deposits are also detected in Lewy body dementia, Down’s syndrome, amyloidosis (Dutch type) and in the Guam
Parkinson-Dementia complex.

Applications:
Suitable for use in ELISA, Immunohistochemisry and Immunoprecipitation. Not suitable for Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (paraffin): Requires protein digestion pre-treatment of paraffin sections using formic acid for 2-3 minutes. (Detects extracellular amyloid beta with senile plaques and vessel amyloid in Alzheimer's disease brain.)
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibody:
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo

Recommended Positive Control Tissue:
Alzheimer's disease brain

Recommended Negative Controls:
I1904-78R: IgG1 Murine Negative Control

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H532Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide consisting of residues 1 - 17 of the amyloid beta protein.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes the human beta amyloid peptide, which is the major protein component of the amyloid cores in neuritic plaques.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kitamoto, T. et al. Lab. Invest. 57: 230 - 236. (1987).