		Technical Data
A2275-85K	APP, phosphorylated (Ser198) (Amyloid beta A4 Protein, APP, ABPP, Alzheimer Disease Amyloid Protein, Cerebral Vascular Amyloid Peptide, CVAP, Protease Nexin-II, PN-II, APPI, PreA4, A4, AD1)

Description:
APP is a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser198 of human APP (KLH).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human APP when phosphorylated at Ser198.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Blomqvist, M.E., et al., Neurobiol. Aging 26(6):795-802 (2005). 2. Yoon, I.S., et al., J. Biol. Chem. 280(20):20140-20147 (2005). 3. Chen, M., et al., Biochem. Biophys. Res. Commun. 330(3):714-721 (2005). 4. Yamamoto, M., et al., Am. J. Pathol. 166(5):1475-1485 (2005). 5. Moir, R.D., et al., J. Biol. Chem. 280(17):17458-17463 (2005).