Technical Data
A2275-85P
APP, NT (Amyloid beta A4 Protein, APP, ABPP, Alzheimer Disease Amyloid Protein, Cerebral Vascular Amyloid Peptide, CVAP, Protease Nexin-II, PN-II, APPI, PreA4, A4, AD1)
Description:
APP is a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).

Applications:
Suitable for use in ELISA, Flow Cytometry, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:10-1:50
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As Reported
Immunogen:
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 29-61 in the N-terminal region of human APP, UniProt Accession #P05067. Species Sequence Homology: monkey
Purity:
Purified by ammonium sulfate precipitation
Form
Supplied as a liquid in PBS, 0.09% sodium azide
Specificity:
Recognizes human APP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Borroni, B., et al., Arch. Neurol. 60(12):1740-1744 (2003). 2.Lleo, A., et al., J. Biol. Chem. 278(48):47370-47375 (2003). 3.Zhou, Y., et al., Science 302(5648):1215-1217 (2003). 4.Murakami, K., et al., J. Biol. Chem. 278(46):46179-46187 (2003). 5.Kim, J.Y., et al., Biochem. Biophys. Res. Commun. 310(3):904-909 (2003).