		Technical Data
A2275-86L	APP (Amyloid beta A4 Protein, ABPP, APPI, Alzheimer Disease Amyloid Protein, Cerebral Vascular Amyloid Peptide, CVAP, PreA4, Protease Nexin-II, PN-II, A4, AD1)

Description:
Accumulation of the amyloid-b peptide (Ab) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The beta amyloid protein precursor (APP) is cleaved by beta secretase, producing a soluble derivative of the protein and a membrane anchored 99aa C-terminal fragment (C99). The C99 fragment serves as substrate for gamma secretase to generate the 4kD amyloid-beta peptide (Ab), which is deposited in the brains of all suffers of Alzheimer’s disease.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Immunohistochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Murine brain lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.1mg/ml

Immunogen:

Synthetic peptide corresponding to aa of human amyloid A4 protein precursor (APP).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

Specificity:

Recognizes human APP. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Ponte, P. et al. Nature 331, 525 (1988). 2. Selkoe, DJ. et al. Annu. Rev. Cell Biol. 10, 373 (1994).