Technical Data
A2275-86Q
APP, Phosphorylated (Thr668) (Amyloid beta A4 Protein, ABPP, APPI, Alzheimer Disease Amyloid Protein, Cerebral Vascular Amyloid Peptide, CVAP, PreA4, Protease Nexin-II, PN-II, A4, AD1)
|
Technical Data |
|
A2275-86Q |
APP, Phosphorylated (Thr668) (Amyloid beta A4 Protein, ABPP, APPI, Alzheimer Disease Amyloid Protein, Cerebral Vascular Amyloid Peptide, CVAP, PreA4, Protease Nexin-II, PN-II, A4, AD1) |
|
|
Description: Amyloid beta (A4) Precursor Protein (APP) is a ubiquitously expressed transmembrane protein that is sequentially cleaved by beta-secretase and gamma-secretase to release extracellular peptides such as the b-amyloid peptides, which are deposited in the brain in Alzheimer's disease. APP exists in several isoforms ranging from 100-140kD, and is phosphorylated on various residues within the extracellular and cytoplasmic domains, effecting the proteolytic processing and secretion of the protein. Threonine 668 can be phosphorylated by cyclin-dependent kinase 5 (cdk5), GSK-3b and JNK, and may play an important role in neurite outgrowth of differentiating neurons. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: CAD cells transfected with wild type vs. T668A mutant APP Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
|
|||||||||||
|
||||||||||||
|
||||||||||||
|
||||||||||||