Technical Data
A2275-87S
APP (Amyloid beta A4 Protein, ABPP, APPI, Alzheimer Disease Amyloid Protein, Cerebral Vascular Amyloid Peptide, CVAP, PreA4, Protease Nexin-II, PN-II, A4, AD1)
Description:
Accumulation of the amyloid-b peptide (Ab) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The b-amyloid protein precursor (APP) is cleaved by b-secretase, resulting in a soluble derivative of the protein and a membrane anchored 99aa carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4kD amyloid-b peptide (Ab), that is deposited in the brain of patients of Alzheimer's disease.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-4ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse or rat brain

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide corresponding to aa653-662 of human amyloid A4 protein precursor (APP) or aa1-10 of the 4K Ab peptide generated by b- and g-secretases.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human APP. Species Crossreactivity: mouse and rat. Species sequence homology: bovine, chicken, guinea pig, porcine, rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ponte P, et al. Nature 331:525-7 (1988). 2. Selkoe DJ, et al. Annu Rev Cell Biol 10:373-403 (1994).