Technical Data
A2277-01
Anaplastic Lymphoma Kinase (Nucleophosmin, p80, ALK, NPM, B23, NPM-ALK)
|
Technical Data |
|
A2277-01 |
Anaplastic Lymphoma Kinase (Nucleophosmin, p80, ALK, NPM, B23, NPM-ALK) |
|
|
Description: The ALK gene is carried by chromosome 2. Upon translocation between chromosome 2 and chromosome 5 [t(2;5}], the ALK gene fuses with the NPM gene. NPM, also known as nucleophosmin or B23, is an ubiquitous nucleolar phosphoprotein involved in the transport of pre-ribosomal components between the nucleolus and the cytoplasm. The chimeric product (NPM-ALK) resulting from t(2;5) translocation is a protein of 80kD with the N-terminal portion of NPM linked to the complete intracellular portion of ALK. ALK is found in many large cell lymphomas. Karpas 299 and SU-DHL-1 lymphoid cell lines strongly express NPM-ALK. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunoprecipitation: 5-10ug/IP reaction Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Positive Controls: T cell lines with the NPM-ALK translocation, including Karpas 299 and TS cells lysates. . Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
|
|||||||||||
|
||||||||||||
|
||||||||||||
|
||||||||||||