Technical Data
A2277-01
Anaplastic Lymphoma Kinase (Nucleophosmin, p80, ALK, NPM, B23, NPM-ALK)
Description:
The ALK gene is carried by chromosome 2. Upon translocation between chromosome 2 and chromosome 5 [t(2;5}], the ALK gene fuses with the NPM gene. NPM, also known as nucleophosmin or B23, is an ubiquitous nucleolar phosphoprotein involved in the transport of pre-ribosomal components between the nucleolus and the cytoplasm. The chimeric product (NPM-ALK) resulting from t(2;5) translocation is a protein of 80kD with the N-terminal portion of NPM linked to the complete intracellular portion of ALK. ALK is found in many large cell lymphomas. Karpas 299 and SU-DHL-1 lymphoid cell lines strongly express NPM-ALK.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 5-10ug/IP reaction
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Controls:
T cell lines with the NPM-ALK translocation, including Karpas 299 and TS cells lysates.
.
Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13G6Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant protein derived from the human ALK protein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes the human ~80kD ALK protein. Crossreactivity with related proteins has not been observed.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.