Androstenedione (ASD) Human, BioAssay™ ELISA Kit
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Competitive immunoenzymatic colorimetric method for quantitative determination of Androstenedione concentration in serum and plasma.
Androstenedione (also known as 4-androstenedione) is a 19-carbon steroid hormone produced in the adrenal glands and the gonads as an intermediate step in the biochemical pathway that produces the androgen testosterone and the estrogens estrone and Estradiol. It is the common precursor of male and female sex hormones. Some androstenedione is also secreted into the plasma, and may be converted in peripheral tissues to testosterone and estrogens.
Androstenedione has relatively weak androgenic activity, estimated at ~ 20% of testosterone. However, serum androstenedione levels often exceed testosterone in both normal and disease states. Secretion and production rates also exceed those of testosterone in women in whom significant extra-adrenal conversion of androstenedione to testosterone occurs. In premenopausal women the adrenal glands and ovaries each produce about half of the total
androstendione (about 3 mg/day). After menopause androstenedione production is about halved, primarily due to the reduction of steroid secreted by the ovary. Nevertheless, androstenedione is the principal steroid produced by the postmenopausal ovary.
Measurement of serum androstenedione provides a useful marker of androgen biosynthesis. Elevated androstenedione levels have been demonstrated in virilizing congenital adrenal hyperplasia. Serum androstenedione levels are also increased in polycystic ovary syndrome, and in case of hirsutism in women. Elevated serum androstenedione levels may also occur in adrenal and ovarian virilizing tumors.
Detection Range: 0-10ng/ml
Sample: 50ul serum
Total Time: ~80 min
1. Microtiter Plate, 1x96 wells
2. Standard S0, (0ng/ml), 1x1ml
3.Standard S1, (0.1ng/ml), 1x1ml
4. Standard S2, (0.4ng/ml), 1x1ml
5. Standard S3, (1.2ng/ml), 1x1ml
6. Standard S4, (4.0ng/ml), 1x1ml
7. Standard S5, (10.0ng/ml), 1x1ml
8. Positive Control, 1x1ml
9. Androstenedione-HRP conjugate, 1x21ml
10. Wash Buffer (10X), (NaCl 160g/l ; Tween-20 g/l, 0.2M Phosphate Buffer, pH 7.4), 1x50ml
11. TMB substrate (H2O2/TMB), 1x15ml
12. Stop Solution (0.15M H2SO4), 1x15ml
Storage and Stability:
Store all components at 4°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.