Technical Data
A2292-23
Angiocidin (S5a, RPN10a, Mcb1)
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Technical Data |
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A2292-23 |
Angiocidin (S5a, RPN10a, Mcb1) |
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Description: Angiocidin (also S5a, RPN10a and Mcb1) is a 41kD (predicted) member of the proteosome subunit S5A family of proteins. It runs anomalously in SDS-page at 55kD. Intracellularly, Angiocidin is a component of the 26S proteosome that degrades ubiquitinated proteins. Extracellularly, it inhibits angiogenesis by binding to collagen and beta 1 integrins. Mouse Angiocidin is 376aa in length. It contains a vWF-A domain (aa5-188), a beta 1 interacting site (aa86-105) and two ubiquitin-interacting motifs (aa211-230 and 282-301). There are four splice variants. Three show a GlyGluArg insertion after Glu254, with two of these showing an additional three aa substitution for aa255-376, and a 45aa substitution for aa321-376, respectfully. A fourth shows a 51aa substitution for aa299-376. Over aa1-254, mouse Angiocidin shares > 99% aa identity with rat and human Angiocidin. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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