Technical Data
A2293-01B
Angiopoietin 1 (Agpt, ang1, Ang1, Ang-1, ANG-1, Angiopoietin-1)
Description:
Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognizes a band above 55kD. This antibody gives a band at 75kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55kD.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
200ul4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide, CNQRRNPENGGRRYNRIQHGQ corresponding to aa21-40 located near the N-terminus of mouse Ang-1.
Purity:
Serum
Form
Supplied as a liquid in PBS, pH7.2, 0.1% sodium azide.
Specificity:
Recognizes mouse Angiopoietin 1 (mAng-1),at ~75kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chade, A. R., Krier, J. D., Textor, S. C., Lerman, A., and Lerman, L. O. (2006) Endothelin-A Receptor Blockade Improves Renal Microvascular Architecture and Function in Experimental Hypercholesterolemia, 17, 3394-3403.