Technical Data
A2293-11A
Angiopoietin 2 (Ang-2, AGPT2, ANGPT2, Ang2) (BSA and Azide free)
Description:
Embryonic vascular system undergoes a series of complex, highly regulated series of events involving differentiation, migration and association of primitive endothelial cells. This process is termed vasculogenesis. A further remodeling of the primitive vascular system forms the mature cardiovascular system. This process is known as angiogenesis (sprouting of new capillary vessels from pre-existing vasculature). Angiogenesis accounts for the formation of vasculature into previously avascular organs such as brain and kidney. Angiogenic activity in the adult is required during normal tissue repair, and for the remodeling of the female reproductive organs (ovulation and placental development). Certain pathological conditions, such as tumor growth and diabetic retinopathy, also require angiogenesis. Study of tumor angiogenesis has led to the identification of several proteins in the angiogenic pathway. Angiopoietin-1 is an angiogenic secreted protein that interacts with endothelial specific Tie-2 receptor. It is primarily expressed in developing endothelial cells. During embryonic development, Ang-1 binds and induces tyrosine phosphorylation of Tie-2. Ang-1 may play a critical role in heart development. A homolog of Ang-1, termed Angiopoietin-2 (mouse and human Ang-2, 496aa; ~85% identity) has recently been identified. It may act as an antagonist for Ang-1 and Tie-2. Ang-1 and Ang-2 have ~60% sequence homology.

Applications:
Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:10,000-1:100,000 using 50-100ng A2293-12 control peptide/well
Western Blot: 1-10ug/ml using ECL. Predicted size of Ang-2 is ~55kD; size may appear higher on PAGE gels due to glycosylation.
Immunohistochemistry: 2-20ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide consisting of 20aa near the N-terminus of the mouse Ang-2 (KLH). Species sequence homology: rat-100%, porcine-85%, human-73% and chicken-55%. Recognizes mouse Ang-2. No significant sequence homology with Ang-1, Ang-3, Ang-4 or ARPs.
Purity:
Purified by affinity chromatography
Form
Supplied as a liquid in PBS
Specificity:
Recognizes mouse Ang-2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Johansson, A. et al., (2005) Endocrinology 146:3463-3470. 1. Maisonpierre C et al (1997) Science 277, 55. 2. Suri S et al (1996) 87, 1171; 3. Davis, S et al (1996) cell 87, 1161