Technical Data
Angpt2 (Angiopoietin-2, ANG-2, Agpt2)
Angiopoietin2 (Ang2 also ANGPT2) is a secreted 66-70kD glycoprotein member of the angiopoietin family of growth factors. It is expressed by endothelial cells, macrophages and skeletal muscle fibers, and binds to the Tie2 receptor on select cell types, including endothelial cells and monocytes. Ang2 has multiple functions. In the presence of growth factors, it promotes angiogenesis in the absence of growth factors, it induces vascular regression. It is generally considered to be an antagonist of Ang1activity. Mature mouse Ang2 is 478aa in length (aa19-496). It contains one coiled coil region (aa159-256) plus a C-terminal Fibrinogen like domain (aa275-495). Mouse Ang2 undergoes covalent oligomerization, forming 140kD homodimers and 280kD homotetramers. The higher order oligomers are strong Tie2 agonists. Over aa68-496, mouse Ang2 shares 87% and 96% aa sequence identity with human and rat Ang2, respectively.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2A12K9Affinity Purified
100ug-20CBlue IceMouseRat
~1mg/ml after reconstitution
Recombinant corresponding to aa68-496 from mouse Angiopoietin2. expressed in CHO cell line.
Purified by Protein A/G affinity chromatography.
Supplied as a lyophilized powder in PBS, trehalose.
Recognizes mouse Angiopoietin2. Species sequence homology: human (87%) and rat (96%).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.