Technical Data
A2293-48B
Angiopoietin-like 3 (ANGPTL3, ANGPT5)
Description:
Angiopoietin-like 3 (ANGPTL3 or ANGPT5), a liver specific protein, is a secreted factor consisting of an N-terminal coiled-coil domain and C-terminal fibrinogen-like domain. ANGPTL3 is structurally similar to angiopoietins, which are vascular endothelial growth factors. ANGTPLs (ANGTPL3, ANGPTL4 and ANGPTL6) have a major impact on lipid and possibly glucose metabolism. These proteins also have important effects on plasma triacylglycerol clearance, adipose tissue lipolysis, and adiposity.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:1000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ANGPTL3 (243-460aa) protein.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k7G4Affinity Purified
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant human ANGPTL3 (243-460aa) purified from E. coli.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% Thimerosal, before the addition of glycerol to 40%.
Specificity:
Recognizes human ANGPTL3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
11. Ono M., et al. (2003) J. Biol. Chem. 278, 41804-41809. 2. Inaba T., et al. (2003) J. Biol. Chem. 278, 21344-21351. 3. Koishi R., et al. (2002) Nat. Genet. 30, 151-157. 4. Kersten S. (2005) Biochem Soc. Trans. 33, 1059-1062.