		Technical Data
A2293-48B	Angiopoietin-like 3 (ANGPTL3, ANGPT5)

Description:
Angiopoietin-like 3 (ANGPTL3 or ANGPT5), a liver specific protein, is a secreted factor consisting of an N-terminal coiled-coil domain and C-terminal fibrinogen-like domain. ANGPTL3 is structurally similar to angiopoietins, which are vascular endothelial growth factors. ANGTPLs (ANGTPL3, ANGPTL4 and ANGPTL6) have a major impact on lipid and possibly glucose metabolism. These proteins also have important effects on plasma triacylglycerol clearance, adipose tissue lipolysis, and adiposity.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:250-1:1000
Optimal dilutions to be determined by the researcher.

Hybridoma:
Derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ANGPTL3 (243-460aa) protein.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1,k	7G4	Affinity Purified

Size		Storage	Shipping	Source	Host
50ul		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~0.5mg/ml

Immunogen:

Recombinant human ANGPTL3 (243-460aa) purified from E. coli.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.02% Thimerosal, before the addition of glycerol to 40%.

Specificity:

Recognizes human ANGPTL3.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

11. Ono M., et al. (2003) J. Biol. Chem. 278, 41804-41809. 2. Inaba T., et al. (2003) J. Biol. Chem. 278, 21344-21351. 3. Koishi R., et al. (2002) Nat. Genet. 30, 151-157. 4. Kersten S. (2005) Biochem Soc. Trans. 33, 1059-1062.