Technical Data
A2293-48C
Angiopoietin-like 3 (ANGPTL3, ANGPT5)
Description:
Angiopoietin-like 3 (ANGPTL3), also known as angiopoietin-5, is a secreted, hepatic angiopoietin-like glycoprotein that is structurally related to angiopoietins. Human ANGPTL3 contains an N-terminal heparin-binding motif, a coiled-coil domain (CCD) that mediates the formation of non-covalent higher-order oligomers, and a C-terminal fibrinogen-like domain that binds integrin v 3. In vivo, ANGPTL3 is proteolytically cleaved between the CCD and the fibrinogen-like domain, generating an N-terminal fragment that retains the inhibitory activity on lipoprotein lipase and a C-terminal fragment that can induce angiogenesis. Full length and cleaved ANGPTL3 circulate in plasma. Mature human ANGPTL3 shares 76% and 83% aa sequence identity with mouse and porcine ANGPTL3, respectively.

Applications:
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human ANGPLT3. The detection limit for rhANGPTL3 is approximately 1ng/lane under non-reducing and reducing conditions. In this format, this antibody shows approximately 20% crossreactivity with rmANGPTL3.
Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human ANGPLT3. The detection limit for rhANGPTL3 is approximately 2.5ng/well.
Optimal dilutions should be determined by each laboratory for each application.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanSheep
Concentration:
As reported
Immunogen:
Sf21-derived rhANGPTL3
Purity:
Purified by human ANGPLT3 affinity chromatography.
Form
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.
Specificity:
Selected for its ability to recognize human ANGPLT3 in direct ELISAs and Western Blots.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.